Majdedin Ghalavand; Fatemeh Parandin; Ehsan Lutfi; Hadi Lotfi; Nafiseh Shourideh Yazdi; Ruhollah Dorostkar; Mahdieh Farzanehpour; Hadi Esmaeili Gouvarchin Ghaleh
Volume 24, Issue 8 , 2022
Abstract
Cancer has always been a severe threat to health and life. Since patients with advanced cancer often have a limited survival time and high treatment expenditures, routine therapies, such as surgery, radiation, and chemotherapy may help them live longer. However, the majority of these individuals cannot ...
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Cancer has always been a severe threat to health and life. Since patients with advanced cancer often have a limited survival time and high treatment expenditures, routine therapies, such as surgery, radiation, and chemotherapy may help them live longer. However, the majority of these individuals cannot afford the excessive cost of care and have short life duration. With the introduction of oncolytic bacteria and viruses, a revolutionary therapeutic technique for the treatment and potential cure of malignant tumors has emerged. Clostridium, Bifidobacteria, Salmonella typhimurium, Listeria monocytogenes, and Bacillus are all oncolytic bacteria. Adenoviruses, Vaccinia viruses, Reoviruses, Herpesviruses, and Coxsackieviruses are all oncolytic viruses. This study aimed to review the current studies on the therapeutic potential of oncolytic bacteria and viruses as an alternate method for cancer prevention and therapy, including both experimental and clinical trials.
Masoumeh Bolandian; Hadi Esmaeili Gouvarchin Ghaleh; Ruhollah Dorostkar; Mahdieh farzanehpour; Bahman Jalali Kondori; Farshad NajjarAsiabani
Volume 24, Issue 2 , 2022
Abstract
Background: Cervical cancer is one of the most common cancers in women throughout the globe. According to reports, currently used cervical cancer therapies are harmful to patients. These treatments have also revealed certain adverse effects that may have a severe impact on the lives of women who are ...
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Background: Cervical cancer is one of the most common cancers in women throughout the globe. According to reports, currently used cervical cancer therapies are harmful to patients. These treatments have also revealed certain adverse effects that may have a severe impact on the lives of women who are afflicted with this kind of cervical cancer. To combat cancer, fewer hazardous anti-tumor therapies are required. Natural products, such as herbal medicine, have been revealed to have a variety of biological impacts. Pistacia atlantica gum extract was tested on the TC1 cell line to see whether it had any anti-proliferative effects.
Objectives: This study aimed to determine the anti-proliferative effect of Pistacia atlantica gum extract on the TC1 cell line.
Methods: Gum extract from dried and milled Pistacia atlantica was analyzed using Gas Chromatography-Mass Spectrometry. The cells were divided into five groups at random, including one for the negative control, one for the positive control, and three for the therapy. Cells from TC1 mice were given the extracts of Pistacia atlantica gum at three different doses (100, 200, and 400 mg/mL). To determine the viability of the cells, reactive oxygen species (ROS) generation, and the concentrations of caspases-3, -8, and -9, researchers used a commercial enzyme-linked immuno-absorbent assay kit.
Results: Results showed that, in comparison to the negative control and most other treatment groups, the Pistacia atlantica gum extract group (400 mg/ml) significantly decreased cell viability (70.138±8.464) and significantly increased apoptosis percentage (71.66±4.041), produced significantly more ROS (15.69±0.799), and significantly increased lactate dehydrogenase release (17.83±0.772) in TC1 cells (P<0.05). In addition, the activity of caspase-8 (0.097±0.007) and caspase-9 (0.065±0.004) increased significantly (P<0.05) in this study.
Conclusion: Our findings indicated that Pistacia atlantica gum extract could drastically decrease cell viability and increase apoptosis in the TC1 cell line, similar to the anti-cancer medication doxorubicin.
